- David J. Ebler, M.D., FACS
- Medical Director – TraumaOne Flight Services, Associate Professor of Surgery, University of Florida College of Medicine – Jacksonville, Department of Surgery – Division of Acute Care Surgery
- Brian Yorkgitis, D.O., FACS
- Associate Medical Director – TraumaOne Flight Services, Assistant Professor of Surgery, University of Florida College of Medicine – Jacksonville, Department of Surgery – Division of Acute Care Surgery
- Andrew C. Schmidt, D.O., M.P.H.
- Deputy Medical Director – TraumaOne Flight Services, Assistant Professor of Emergency Medicine, University of Florida College of Medicine – Jacksonville, Department of Emergency Medicine
- Phyllis L. Hendry, M.D., FAAP, FACEP
- Deputy Medical Director – TraumaOne Flight Services, Professor of Emergency Medicine and Pediatrics, University of Florida College of Medicine – Jacksonville, Department of Emergency Medicine and Pediatrics